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KMID : 0603820120180010079
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Journal of Experimental & Biomedical Science
2012 Volume.18 No. 1 p.79 ~ p.82
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Prevalence of Multi-drug Resistant Acinetobacter baumannii Producing OXA-23-like from a University Hospital in Gangwon Province, Korea
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Jang In-Ho
Lee Gyu-Sang
Choi Il
Uh Young
Kim Sa-Hyun
Park Min
Woo Hyun-Jun
Choi Yeon-Im
Kim Jong-Bae
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Abstract
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Acinetobacter infections are of great concern in clinical settings because of multi-drug resistance (MDR) and high mortality of the infected patients. The MDR Acinetobacter baumannii has emerged as a significant infectious agent in hospitals worldwide. The purpose of this study was to determine for molecular characterization of MDR A. baumannii clinical isolates obtained from the Wonju Christian Hospital in Gangwon province of Korea. A total of seventy nonduplicate A. baumannii isolates were collected from the Wonju Christian Hospital in Korea from March to April in 2011. All of the MDR A. baumannii isolates were encoded by blaOXA-23-like gene and all isolates with the blaOXA-23-like gene had the upstream element ISAba1 to promote increased gene expression and subsequent resistance to carbapenem. 16S rRNA methylase gene (armA) was detected in 44 clinical isolates which were resistant to amikacin, and phosphotransferase genes encoding aac(3)-Ia and aac(6")-Ib were the most prevalent. A combination of 16S rRNA methylase and aminoglycosidemodifying enzyme genes (armA, aac(3)-Ia, aac(6")-Ib, and aph(3")-Ia) were found in 31 isolates. The sequencing results for the quinolone resistance-determining region (QRDR) of gyrA and parC revealed the presence of Ser (TCA) 83 Leu (TTA) and Ser (TCG) 80 Leu (TTG) substitutions in the respective enzymes for all MDR. Molecular typing for MDR A. baumannii could be helpful in confirming the identification of a common source or cross-contamination. This is an important step in enabling epidemiological tracing of these strains.
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KEYWORD
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Multi-drug resistant Acinetobacter baumannii, blaOXA-23-like, ISAba1, 16S rRNA methylase gene, Aminoglycoside-modifying enzyme, Quinolone resistance-determining region
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